In a paper presented at the 30th Annual San Antonio Breast Cancer Symposium, at Texas, from December 13-16, 2007, Dr.Keizo Sugimachi, MD, President of the Kyushu Central Hospital in Fukuoka, Japan, has reported that his team successfully used adipose-derived stem cells and regenerative cells for superior breast reconstruction after partial mastectomy.
The researchers performed the study on metastasis-free women who had undergone partial mastectomy for breast cancer. The adipose tissue was harvested from the women by conventional tumescent liposuction under anesthesia. In order to obtain a suspension of Adipose-Derived Stem Cells (ADSCs), the Celutionâ„¢ System (Cytori Therapeutics) was used to process one part of the adipose tissue. The other part was purified and mixed with concentrated ADSCs, and injected into the breast incision. At the 3rd and 6th months after the stem cell augmented reconstruction procedure, the researchers measured the thickness of the reconstructed portion of the breast using 2D ultrasound. They observed a significant improvement in the breast tissue thickness at final assessment, compared to baseline. The procedure was also deemed to be safe and well tolerated, without any rejection or immune response. However, the researchers recommended further studies to evaluate the safety and efficacy of the novel approach.
To improve the cosmetic outcome and to correct certain sequelae associated with the conservative treatment of breast cancer, Missana and colleagues (European Journal of Surgical Oncology, 2007) employed a technique known as autologous fat transfer during reconstructive breast surgery. The patients were followed up for a mean period of 11.7 months and the feasibility of fat grafts and detection of any abrasion in the breast was monitored with pre- and post-operation imaging. The results demonstrated that 86.5% patients showed good improvement with this technique, while another 13.5% had moderate improvement in outcome. The researchers concluded that the technique, which is simple, safe and reproducible, could be implemented as a standard procedure for obtaining a refined and superior result during breast reconstruction.
Rigotti et al (Plastic and Reconstructive Surgery, 2007) conducted a study on patients undergoing treatment for radiation side-effects, to determine the presence of residual ADSCs in purified lipoaspirates, and to evaluate the efficacy of this lipoaspirate transplantation in treating radiotherapy tissue damage. After low-invasive computer-assisted injection of the purified lipoaspirates from healthy tissues of the patients, the scientists evaluated the treatment outcomes through symptom classification, cytofluorimetric characterization, and ultrastructural evaluation of the targeted tissue. The study results showed a progressive regeneration and improvement in neovessel formation, better tissue hydration, and improvement in symptoms in all the patients.
In plastic and reconstructive surgeries, adipose cells are being increasingly used as a filler-material to repair soft tissue defects due to traumatic injuries, tumor resection, and congenital defects. The adipose-derived stem cells are considered to have great potential in reconstructive surgery, and also in the treatment of conditions such as cardiovascular diseases and orthopedic disorders.
Recently, the Celutionâ„¢ System received 510(k) regulatory clearance from FDA’s Center for Devices and Radiological Health (CDRH), to harvest, filter, and transfer autologous adipose stem cells at the point-of-care during the same surgical procedure. The use of the Celutionâ„¢ System to retrieve the stem cells from adipose tissue boosts the efficacy of the autologous stem cell graft technology. Given that current reconstruction techniques do not guarantee optimal post-surgical appearance and shape to the breast, reconstruction using adipose stem cells is a definite step forward in perfecting breast conservation therapy.
About Cytori Therapeutics – Cytori Therapeutics, headquartered in San Diego, CA, has developed the Celutionâ„¢ System to process autologous adipose stem cells and regenerative cells. Several clinical trials are planned and currently on to investigate the use of adipose derived stem cells in cardiovascular diseases, spinal disc degeneration, and reconstruction surgery.
1. Adipose-Derived Stem Cells Show Promise in Breast Reconstruction, Results Reported at the 30th San Antonio Breast Cancer Symposium. Press Release. Cytori Therapeutics. Last Accessed on December 18, 2007.
2. Missana MC, Laurent I, Barreau L, Balleyguier C. Autologous fat transfer in reconstructive breast surgery: indications, technique and results. European Journal of Surgical Oncology. 2007 August;33(6):685-90.
3. Rigotti G, Marchi A, GaliÃ¨ M et al. Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plastic and Reconstructive Surgery. 2007 April 15;119(5):1409-22.
4. About Cytori Therapeutics. Cytori. Last Accessed on December 18, 2007.